Quinalizarin, a specific CK2 inhibitor, reduces cell viability and suppresses migration and accelerates apoptosis in different human lung cancer cell lines.

BACKGROUND: Protein kinase CK2 is widely expressed in eukaryotic cells, and plays an important role in cell proliferation, migration, apoptosis, etc. The aim of the current study is to explore how Quinalizarin, a specific CK2 inhibitor, affects the cell proliferation, migration, and apoptosis of different pathological and genetic types of human lung cancer cell lines. MATERIALS AND METHODS: MTT assays were performed to evaluate the cell viability after being treated by Quinalizarin. Transwell migration assays were used to assess whether Quinalizarin could suppress cell migration. Flow cytometry was employed to test the apoptosis rate of different cells. RESULTS: After being treated by Quinalizarin, the viability of different pathological types of lung cancer cells (H446, H460, A549) were significantly suppressed in a time and dose‑dependent manner. More interestingly, in a serial of human lung adenocarcinoma cell lines with different epidermal growth factor receptor (EGFR) mutation status, Quinalizarin was shown to have a much better ability to reduce the viability of cells with EGFR sensitive mutation than those with resistance mutations. Meanwhile, we also found that the cell migration of different pathological types of lung cancer cells (H446, H460, A549) was significantly decreased by Quinalizarin dose‑dependently. In addition, the apoptosis rates in those cells were proved to be increased after exposed to Quinalizarin. CONCLUSIONS: Quinalizarin, the specific CK2 inhibitor, could reduce cell viability with emphasis on adenocarcinoma cells harboring EGFR sensitive mutation, suppresses migration, and accelerates apoptosis in different human lung cancer cell lines.

Tear Secretion and Tear Film Function in Diabetics.

In the present study, the evaluation of the amount of the tear production, the stability of the tear film and the condition of the conjunctival surface by the use of impression cytology in the diabetics and the non diabetic individuals was done to detect the possible tear film anomalies in the Type 2 diabetic patients. We performed Schirmer 1 test, TFBUT (Tear film break up time) and CIC (Conjunctional Impression Cytology) on each subject in both groups. The mean values for Schirmer 1 test, TFBUT were significantly decreased in diabetes patient as compared to healthy controls. The CIC revealed pronounced degree of metaplasia with loss of goblet cells in diabetic patients. We found decreased tear production, unstable tear film and squamous metaplasia in diabetic patients.